rapidly identifying potential CRISPR/Cas9 off-target sites (part one)

Before we can score segments in the genome having a small number of mismatches to a CRISPR for their off-target risk, we must first find these segments. Searching for every possible mismatch permutation proves computationally expensive, so we apply the following heuristic: We only search for mismatches in the top positions relevant to CRISPR efficiency. […]